Optimization of DNA extraction from brown algae (Phaeophyceae) based on a commercial kit

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Journal pf phycology Volume 46, Issue 3
Alodie Snirc, Thomas Silberfeld, Jacques Bonnet, Annie Tillier, Sophie Tuffet, Jian-Sheng Sun
http://onlinelibrary.wiley.com/doi/10.1111/j.1529-8817.2010.00817.x/abstract
Large-scale DNA molecular studies require reliable and efficient tools for DNA extractions. However, for some plant species and brown algae, isolation of high-quality DNA is difficult. We developed a novel method for isolating high-quality DNA from the …Read More

Chain and conformation stability of solid-state DNA: implications for room temperature storage

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Nucleic Acids Research Volume 38, Issue 5
Bonnet J., Colotte M., Coudy D., Couallier V., Portier J., Morin B., Tuffet S.
https://academic.oup.com/nar/article/38/5/1531/3112491
There is currently wide interest in room temperature storage of dehydrated DNA. However, there is insufficient knowledge about its chemical and structural stability. Here, we show that solid-state DNA degradation is greatly affected by atmospheric water …Read More

Simultaneous assessment of average fragment size and amount in minute samples of degraded DNA

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There is currently no method allowing routine characterization of minute amounts of degraded DNA samples such as those encountered in forensic science, archived tissues, ancient DNA, extracellular or stool DNA or processed food. Here, we describe and directly validate such a method based, on one hand, on a generalized DNA random fragmentation model and on …Read More

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