English
Français
RNAshell®
RNAshell® minicapsules preserve RNA from its major degradation factors by keeping this molecule in an anoxic and anhydrous atmosphere. RNA samples can thus be stored at room temperature from short to very long term. Identified by a laser-marked unitary 2D Datamatrix code, the RNAshell® minicapsules ensure the complete, permanent and inviolable traceability of the RNA samples.
RNAshell® technology is based on the confinement of purified and desiccated RNA molecules protected from alteration factors (water, oxygen, light, xenobiotics) under an inert atmosphere (argon, helium <1ppm oxygen and of water) inside tight stainless steel minicapsules (about 0.7 mL) that can be placed in 96-well microplate-type racks in standard SBS (Standard of Biological Society) format.
- The RNAshell® minicapsule, consisting of a stainless steel case and a stainless steel cap, is hermetically sealed by laser welding. It contains a glass insert.
- The useful volume of the minicapsule is 200 μL
- The 2D Datamatrix code is engraved on the bottom of the capsule for easy, unique and automated identification using a 2D Datamatrix reader.
- The tightness of each capsule is controlled by a helium test based on mass spectrometry.
- DNAshell® minicapsules are stored in their microplates at room temperature avoiding temperature or humidity control devices.
- For RNA recovery, the stopper is perforated with a disencapsulator (see accessories) and the sample is rehydrated by simply adding water or a buffer solution.
RNAshells® performance
Stability and quality of RNAshell® RNAs at room temperature
From Fabre A-L et al., 2013, European Journal of Human Genetics – Nature
A: The messenger RNA purified and stored in RNAshell® for 24 weeks is analyzed by agarose gel electrophoresis. The measurement of the intensity of the mRNA band by Bio1D software shows no degradation over 6 months.
B: Total RNA conserved in RNAshell® for 2 years is analyzed by agarose gel electrophoresis and bioanalyzer. No significant evolution of the RIN nor of the 28S / 18S ratio is observed during the 2 years of conservation. However, the RNA, in dry form, exposed to ambient air deteriorate as early as 2 weeks, highlighting the need for an anhydrous and anoxic atmosphere for reliable preservation.
Long-term stability of RNAshell® RNAs – accelerated aging by heating
From Fabre A-L et al., 2013, European Journal of Human Genetics – Nature
RNAshell® are either heated directly at 90 °C for up to 242 h (A) or opened and heated at 90 ° at 50 % relative humidity to study the effect of the atmosphere (50% RH = South of France in summer) (B).
Five hundred nanograms of total RNA are analyzed by agarose gel electrophoresis.
The proportion of intact 28S RNA is measured with the Bio1D software and indicated for each point of the kinetics. No significant degradation is observed up to 48 h at 90 ° C in RNAshell®, heating time corresponding to 30 years at room temperature.
On the other hand, ribosomal RNAs exposed to 50 % RH degrade as early as 3 hours at 90 ° C, confirming the need for air protection for safe storage.
Complete recovery of RNAshell®-conserved RNA samples
From Liu X et al., 2015, Biopreservation and Biobanking
The recovery efficiency of the RNAshell® RNAs is constant during the 16 days of the kinetic unlike the RNAs kept in solution.
Stability of standard RNAs for quality internal controls maintained in RNAshell® and compatibility with molecular analyzes by quantitative PCR
From Cayuela J-M et al., 2015, Clinical Biochemistry
Stability of BCR-ABL1 quality internal controls maintained in RNAshell® even after acceleration of aging to 100 years.